Kuchmerovs'ka T. Biological action of vitamin PP and its derivatives in nervous system

Brain and its subcellular fractions, sciatic nerve and liver of rats. The methods: ultracentrifugation, gel-electrophoresis, chromatography, spectrophotometry, enzymatic methods. The elucidation ofmechanisms of biological action of vitamin PP and its derivatives in nervous system and NAD participation in serotonin-, dopamine- and GABA-ergic systems functioningin norm and under experimental models of several pathologies. The existence of glicolipoproteins complexes of NAD-binding sites is suggested. It was identified NAD-binding protein of synaptic membranes and established its molecular weight. Involvement of synaptic vesicules of nerve endings inrealisation of NAD presynaptic modulating effects was determined. NAD-modulating system was shown to be present in nervous ending. Its function is realized through concentration-dependent modulation of serotonin, dopamine and GABA release and uptake by NAD under experimental models (PP-hypovitaminosis, Parkinson's disease, diabetic neuropathy). The results are important in theoretical aspect and for practical utilization vitamin PP and its derivatives (nicotinoyl-GABA) under Parkinson's disease and diabetic neuropathy. Field of application - scientific institutions of medicaland biological type.