Kapralov O. The role of vitamin E in the processes of functioning of rat liver nuclei and mitochondria

The goal of our study was the investigation of tocopherol's role in functions of liver cell nuclei and molecular mechanisms of action of this vitamin and tocopherol-binding proteins on nuclear acid synthesis in nuclei and mitochondria. Among the methods of investi gation were the binding of tocopherol with isolated nuclei, mitochondria and their fractions, and also the studying of RNA- and DNA-polymerase activities of these organelles. It was found, that alfa-tocopherol was bound with nuclear membrane, chromatin and nuclear matrix. The action of tocopherol on RNA- and DNA-polymerase activity of cell nuclei and mitochondria was promoted by the addition of specific tocopherol-binding proteins present in these organelles. PKC-, cAMP-, and Ca-dependent mechanisms were able to be involved in the action of alfa-tocopherol on these processes.