Platonova T. Protein-protein interaction under overmolecular fibrin clot structure formation in coagulation system

The object of investigation is the coagulation system proteins taking part in fibrin clot formation process. The aim is: study of the coagulation system components interactions that impose the self-assemblingfibrin process and fibrin derivates influence on haemostasis system regulation mechanisms. The native fibrin beta-C-domain has been purified, and it's role in DB center of fibrin polymerization formation has been found out. The hypothesis about interaction of the EB-DB centers of the fibrin polymerization has been confirmed. The existing of cross-bond of EA-DB centers of the fibrin polymerization has been shown. In first nonenzymatic prothrombin activation by fibrin E-fragment was observed. It has been shown that such prothrombin activation can be one of the possible reoclusion mechanisms. The important role of prothrombin kringles and N-terminal sites of fibrin E fragment beta-chains in interaction of prothrombin with E-fragment has been determined. The diagnostic kits accompained to these test were developed using purified snakes' venom enzymes. These diagnostic tests allow to characterize the haemostasis system at disseminated intravascular coagulation. Field of application: scientific and educational institutes of medical and biological type.