Lugovskoy E. Molecular mechanisms of fibrin formation and fibrinolysis.

Object for the research: fibrinogen, fibrin desAA, fibrin desAABB, fibrin D-dimer. The main aim of the investigation: the search and the study of the functional role of unknown fibrin polymerization sites and the creation of new model of fibrin network formation which are the framework of thrombus; production of monoclonal antibodies specific to main product of fibrinolysis - D-dimer to investigate its antigenic structure and the usage of this monoclonal antibodies for D-dimer quantification in human blood plasma. The methods of the research: physical-chemical, biochemical and immunochemical ones. Theoretical and practical results: the experimental data were obtained which testify to the existence of unknown polymerization sites in human fibrin molecule. These sites function at two stages of fibrin polymerization - protofibril formation and protofibril lateral association. Monoclonal antibody (III-3b) to human fibrin D-dimer has been obtained which react specifically with D-dimer without cross-reactionwith fibrin(ogen); the possibility of the usage of monoclonal antibody III-3b has been shown for D-dimer quantification in human blood plasma and for the creation of appropriate immunotest-system for diagnosis of different diseases - results of blood coagulation system activation. Field of application: biomedical institution.