Kozhukhova N. Molecular markers in maize (Zea mays L.) genome research

The dissertation contains theoretical and experimental material on molecular-genetic polymorphism analysis of maize genome, development of marker systems and usage them in maize genetics and plant-breeding researches. Analysis of SSR-loci allelic composition for maize genotypes of Ukrainian and world breeding was realized, 20 SSR-markers test-panel with high discriminatory ability was created. Verification of lines and hybrids genetic purity by these markers was carried out. Differentiation, authentication and registration as genetic formulas of maize genotypes were conducted. DNA-typing database of lines and hybrids cultivated in Ukraine was created. Correlations of genetic distances values between lines on DNA-typing data and heterosis level of proper hybrids were analyzed. SSR-loci allelic composition of lines and hybrids were defined and models formulas related to combination ability of lines were formed. Locus of resistance to fusarium rot was marked and located on maize chromosome 1. STS-marker RGA11 on 18,3 cM distance is developed for using in breeding for selection of maize resistant genotypes.