Shatursky O. The mechanisms fоr the channel-formіng proteins interaction with lipid bilayer of artificial membrane and ion-conducting properties of the channels they formed

Оbject: Animal and bacterial protein toxins and cellular proteins (L-protein of the nervous cell cytoplasm and ClC-1 chloride channel of muscle cell sarcolemma). Aim: the determination of the mechanisms for channel creation and properties of the channels formed by purified pore-forming proteins in the lipid bilayer of artificail membrane and the influence of natural and artificial factors that affect those channels formation and functioning. Меthods: biochemical, electrophysiological, molecular-biological, physicochemical. The reconstruction of protein neurotoxins into bilayer lipid membranes ( alpha-latrotoxin, alpha-latroinsectotoxin, dalta-latroinsectotoxin of black widow spider Latrodectus mactans venom) and cytolitic toxins ( betha- and tetha-toxins of bacteria Clostridium perfringens, RTX toxin of anemone Radianthus macrodactilus), and also L-protein of bovine brain nerve cell cytoplasm and ClC-1 chloride channel of rat skeletal muscle sarcolemma allowed to determine the mechanisms of those proteins channel creation in the bilayer membrane and the role passive ionic transport through these channels plays in biochemical procecces that influence the nerve-muscle conduction and/or cause the lysis of the target cells. The results obtained created preconditions necessary for creation of means that prevent the development of pathological influence of neurotoxins and cytotoxins by the thiazole derivative DMHT block of ionic current across chennels those toxins had formed. Scope - preferable for the organizations of medical and biological expertize.