The dissertation work is devoted to development of complex biotechnological systems of creation the new forms and clonal micropropagation of essential oil plants (lavender, sage, coriander, fennel, anise, yarrow, rose, geranium) based on investiga-tion of features of isolated organs, callus tissues cultivation and morphogenesis induc-tion in vitro. The conditions of callus cultures obtaining from different explants were opti-mized and peculiarities of exogenous and endogenous factors effects on the callu-sogenesis efficiency of studied essential oil plants have been detected. For the first time the methods of plant regeneration from calluses of different passages for more than fifty cultivars and samples of lavender, sage, coriander, fennel, anise, geranium, yarrow have been developed. The peculiarities of genotype, hormonal composition of culture medium, type of explant and number of passage influence on the morphogene-sis induction have been determined. The means, which permit to increase the fre-quency of organogenesis and somatic embryogenesis of callus cultures and to regener-ate the plants during 1-3 years have been elaborated. The peculiarities of abiotic stress factors effect at calluses with and without morphogenesis and zygotic embryos have been revealed. For the first time the selective systems for screening forms in vitro with resistance to salinity, osmotic and low temperature stress have been elaborated for lav-ender, sage, geranium, coriander. Methods of clonal micropropagation for lavender, geranium, yarrow, fennel, and sage have been worked out. It was shown the influence of cultivar, medium composition, explant disposition on the shoot and cycle of propa-gation on the propagation efficiency. Under analysis of regenerants from lavender, sage, geranium, coriander, yarrow and fennel calluses the somaclonal variability by morphology and economic valuable characteristics have been determined, and perspec-tive for breeding forms have been revealed.
