Zelenyanska N. Scientific substantiation and development of modern technology in culture of grapevine planting

The thesis is dedicated to development of new progressive technology in culture of grapevine planting including technology for fast grapevine propagation by using of methods for in vitro culture. The technology has been developed, theoretically based and proposed for production to increase output of grafted grape seedlings from nursery. It is based on using of new biological active preparations - Radifarm, Bioglobin, Kornevin, Ukorenitel, Charkor, El-1, Potasium humate Ecoorganics and Rost-concentrate; on water holding substrates for open and close methods of stratification and conservation of grafts; on grafting waxes "Norsk Wax" and "Shar" or photodisruption films "Baddy Tape", "Medifilm", "Professional Grafting Tape" and "Shar" to isolate apical parts of grafts from drying up, on ground mulching in nursery by using of combined 30 mkm film of white color. Highly effective biotechnological scheme has been carried out for fast production and receiving of grapevine planting free from viral and bacterial infection in vitro culture. It is based on using of ecological safety systems of sterilization for initial explants, on using of universal nutrients with minimum phyto-hormones content in culture and micropropagation proper, on complex of methods for planting of grape microcuttings and using of structural nutrients on the basis of agroperlite.