Grabovskyi S. Adaptogenic effect of biologically active substances of spleen preparation at stress in animals before slaughter

Object - metabolic processes and functional status of immune system cells in the body of laboratory and farm animals under stress before slaughter and under effect of the adaptogenic action preparation. Purpose - to determine the influence of spleen preparation biologically active substances on the enantiostasis, immune function of the laboratory and farm animals body under pre-slaughter stress and to receive effective adaptogenic action preparation against stress in animals in pre-slaughter period. Methods: biochemical, immunological, cytological, histological, morphological, physicochemical, zootechnical, statistics. The novelty: For the first time received information about the features pre-slaughter state laboratory and farm animals, as well as the anti-stress and immunostimulate effect of "Spleen Active" preparation, obtained from pig's spleen. By the mass spectrometer analysis has been shown in this preparation high concentration of the main ingredients affected as immunostimulators and antistressors for animals before slaughter: phospholipids, arginine and polyamines. The influence of biologically active substances of the preparation, which were reduced the stress negative effects, on certain blood biochemical indices and functional parameters of immune status in different species animals under pre-slaughter stress was founded. Cortisol level was significantly changed in the rats' blood plasma depending on the priority of animal removing from the cage: level was increased by nearly 1.5 times in rats that were removed as the fourth and the fifth animal from each cage. While the cortisol's level increased by nearly 5 times in rats that were removed from each cage as the fifth (last) ones comparing to such level in rats removed as the first ones. The adrenocorticotropic hormone level in blood plasma of the bulls before setting at preparatory period was lower comparing with the bulls immediately before their transportation to slaughterhouse. For the first time was founded that bulls were experienced more stress before transport to slaughterhouse than before begining of the experiment. For the first time was founded that the spleen preparation using before slaughter lowers of rats female apoptotic splenocytes, but in males, in contrast, level of apoptotic spleen cells increases.