Melnyk A. Characteristics of Metabolism of Sulfur-Containing Amino Acids and Hydrogen Sulfide and Their Relations with the State of Cardiovascular System (experimental study)

Object - sex differences in the metabolism of sulfur-containing compounds and their relationship to the state of the cardiovascular system in norm and with hyperhomocysteinemia; aim – to clarify the sexual characteristics of the metabolism of sulfur-containing amino acids and hydrogen sulfide in rats, to assess their relationship to the state of the cardiovascular system in norm and with hyperhomocysteinemia, and on this basis to experimentally substantiate new approaches to the correction of cardiovascular pathology; methods – biochemical, immuno-enzymatic, molecular-genetic, electrophysiological, functional, flow cytometry, pathophysiological, pharmacological, statistical; results – conducted studies have revealed the sexual characteristics of the metabolism of homocysteine, cysteine and hydrogen sulfide in the liver, myocardium and aorta, to evaluate their contribution to the formation of the sexually-associated pathology of the cardiovascular system, and on this basis to justify new approaches to the correction of heart and vascular diseases in individuals of different sex; for the first time it was found that the level of sex hormones, the concentration of homocysteine, cysteine and H2S in the blood are factors that affect the nature of the metabolism of H2S in the cardiovascular system of male and female rats; revealed the sexual characteristics of biochemical and functional parameters of the state of the myocardium and aorta of rats that are determined not only by the type and level of sex hormones, but also by differences in the metabolism of H2S in the cardiovascular system; for the first time, the half-specific effect of H2S metabolism modulators on the biochemical and functional parameters of the myocardium and aorta; for the first time, sex differences in the effect of prolonged administration of thiolactone homocysteine to the metabolism of sulfur-containing amino acids and H2S, the biochemical and functional parameters of the heart and aorta of rats; it was found that with experimental hyperhomocysteinemia, the administration of polyphenolic substances reduced the disturbances in the exchange of sulfur-containing amino acids and H2S in the organs of the rats, accompanied by cardio- and vasoprotective effects; branch – medicine.