Lisoviy M. Theoretical and applied principles of coniferous species propagation in vitro

The object of the study – conifer species (Pinus sylvestris L., Larix decidua Mill., Abіes alba Mill., Рісеа abies (L.) Karsten., Taxus baccata L., Metasequoia glyptostroboides Hu & Cheng та Thuja occidentalis L.) and their рlants-regenerators, obtained by reproduction in vitro. Тhe purpose – to develop theoretical, applied and methodical principles for the propagation of conifer species through their micropropagation in vitro. Methods of research – general scientific, biotechnological, laboratory, mathematical and statistical. Scientific novelty – the influence of concentration and exposure of three chemical reagents (AgNO3, NaClO and HgCl2) on the efficiency of sterilization and viability of explants was established; the methodological principles of the classification of explants of conifer species for qualitative indicators of initiation ("unsatisfactory initiation", "satisfactory initiation" and "good initiation") have been developed under the influence of modified nutritional media by auxins and cytokinins; the influence of growth stimulants on the processes of the formation of the adventitious microshoots in the clones of conifer species in vitro was determined; a qualitative assessment of the degree of rhizogenesis of regeneration plants of conifer species in vitro; optimal terms of cultivation of explants in the stages of initiation and rhizogenesis are determined and theoretical and actual coefficients of micronucleus multiplication of the studied species are established. Practical significance – the developed reference models of methods of micropropagation of conifer species is a methodological basis for implementation of conservation and reproduction of genetic resources of investigated plants and the production of their selectively valuable and genetically stable planting material. The obtained results deepen knowledge about the passage of the basic processes of morphogenesis of explants in micropropagation. Field of use: forestry.