Raietska Y. Biochemical mechanisms chemical burns of the esophagus.

The dissertation is devoted to find out the role of proteolysis processes in molecular-biochemical mechanisms of injuries and healing of esophageal mucosa under conditions after chemical burn (ECB). The results deepen existing views on the functioning of biochemical mechanisms in the development of ECB. An increase of the level of pro-inflammatory cytokines after burns was established against the background of anti-inflammatory cytokine deficiency, which was accompanied by an increase in IgG concentration and the content of medium and low molecular weight circulating immune complexes. Changes in the cytokine profile coincide with the activation of proteolysis and indicate a slowing of wound healing. Significant disturbances of the oxidation-antioxidant equilibrium have been revealed, namely the increase of the level of lipid and protein oxidation products and the dysregulation of antioxidant enzyme systems, which leads to changes in the activity of proteins and their inhibitors under the action of chemical burns of the esophagus. Changes in the proteolytic profile of serum and quantitative changes in the content of protein fractions by ECB were shown. There was a decrease in the content of albumin fraction, an increase in the content of globulin fraction relative to control. The increase of α2-macroglobulin level and the activity of serine and metalloproteinases in blood plasma were found. It has been proved that proteolytic degradation of esophageal tissue proteins occurs during esophageal burns, which consists in changing the ratio of quantitative content of protein fractions, an increase of TIMP-1 content, growth factor of fibroblasts, changes in polypeptide pull and all types of matrix metalloproteinases (MMPs), which influences the accumulation of protein malfunctions and malfunctions proteolysis system.