Векліч Т. The mechanisms of effects of calixarenes on cation-transporting ATP hydrolases and calcium homeoctasis of the myometrium

The thesis is devoted to the study of biochemical and physicochemical mechanisms of effects of calix[4]arenes on membrane-bound cation-transporting electroenzymes of smooth muscle cells and on non-enzymatic hydrolysis of ATP. Therefore, the study of the effects of calix[4]arenes on the enzymatic activities of membrane-bound electroenzymes: Mg2+,ATP-dependent calcium pumps of the plasma membrane and sarcoplasmic reticulum, Mg2+,ATP-dependent sodium pump of the plasma membrane, and on non-enzymatic hydrolysis of ATP, are considerably interesting in both theoretical and practical aspects. In experiments carried out with the plasma membrane fraction of pig smooth muscle cells treated with a solution of digitonin (0.1 mg / ml) and with a suspension of rat myocytes, the effects of calix[4]arenes on Ca2+,Mg2+-ATPase, Na+,K+-ATPase and Mg2+-ATPase activities in the plasma membrane, Ca2+,Mg2+-ATPase activity of the sarcoplasmic reticulum of myometrial cells, on Ca2+-transport activity of Ca2+,Mg2+-ATPase of the plasma membrane, Ca2+ homeostasis in myocytes and on non-enzymatic hydrolysis were investigated. It was found that calix[4]arenes C-99 and C-107 had membranetropic properties and could indirectly affect membrane enzymes. It was shown that calix[4]arene C-107 could be built into membrane and penetrate through the membrane.