In recent years, there has been a significant increase in interest in the study of morpho-functional features of the internal male genitalia, both in normal and pathology, in particular in circulatory disorders in them. Without effective treatment of circulatory disorders of the genitourinary system, which have close topographic and anatomical relationships and functional connections, inflammatory phenomena and serious consequences occur. Moreover, andrologists in inflammatory diseases of both the small and urinary system and urinary tract, almost constantly diagnose structural and functional changes in the seminal vesicles.
In the available literature, there are no comprehensive studies of structural and functional changes in the components of seminal vesicles in circulatory disorders of the reproductive system, as well as in terms of its elimination, which emphasizes the relevance of this study.
The study aimed to investigate the peculiarities of the structure of seminal vesicles in experimental animals and changes after circulatory disorders of the reproductive system with different duration using general histological techniques, immunohistochemistry and electron microscopy.
Materials and methods of research. The object of the study was 115 tissue samples of seminal vesicles of laboratory adult rats, which were divided into 5 groups: 1st control group; 2nd group after modelling of circulatory disorders of the reproductive system with a duration of 7 days; 3rd after modelling of circulatory disorders of the reproductive system with a duration of 20 days; 4th group after theremodelling of circulatory disorders of the reproductive system with a duration of 7 days; 5th after theremodelling of circulatory disorders of the reproductive system with a duration of 20 days.
For the first time, a complex morphological, general histological, immunohistochemical study of the fine organization of the structural components of the seminal vesicles of rats in circulatory disorders of the reproductive system. Under the conditions of modelling circulatory disorders of the reproductive system with a duration of 20 days in seminal vesicles, destructive changes in the wall and epithelium of seminal vesicles are observed, but aftertheremodelling of disorders with a duration of 7 days, regenerative-compensatory restoration of structural components of the wall occurs. vesicles, but after circulatory disorders of the reproductive system with a duration of 20 days in the simulation, there was an increase in degenerative and destructive processes in seminal vesicles, which in the remodelling with a duration of 20 days are very slow, recoverable, but not fully restored.
For the first time, a comparative ultramicroscopic study using electron microscopy was shown, which showed that in the simulation of circulatory disorders of the reproductive system with a duration of 7 days, the general organization of seminal vesicles changes. After ligation of the spermatic cord for 20 days, the integrity of the membranes of the epithelial cells of the seminal vesicles is disrupted, and the plasma membranes lose their structure and typical two-layer structure and become fragmented. Under the conditions of modelling of circulatory disorders of the pelvic organs, there is significant destruction of the epithelial component of seminal vesicles, which increases from the 7th to the 20th day of the experiment and correlates with the degree of damage to the hemomicrocirculatory tract.
After a comprehensive immunohistochemical study using monoclonal antibodies to Ki-67, CD-34 and VEGF, for the first time there was a change in the expression of Ki-67 and CD-34 in the nuclei of epitheliocytes and modification of VEGF distribution in the structural components of seminal vesicle villi of test and control samples. and the distribution of these markers in circulatory disorders in the reproductive system, and not only the level of their expression.
The results of the study help to expand the understanding of the patterns of structural adjustment of seminal vesicles in circulatory disorders of the reproductive system and predict the consequences, circulatory disorders,and possible repair aftertheremodelling of these disorders with adifferent duration on the structural components of the seminal vesicle wall. The obtained data will establish the possible duration during which the influence of circulatory disorders in the simulation leads to morphological changes in the structural components of the seminal vesicle wall, which we can predict and correct by the remodelling, which affects the reparative processes occurring in seminal vesicles in the remodelling of circulatory disorders, taking into account the duration
