Yavorovska V. Thiacalix[4]arenes – protectors of ATPase activity of myosin of myometrium from the influence of heavy metal cations.

Various environmental factors can cause the impaired contractile function of the myometrium, including heavy metal cations. They represent a large group of pollutants, among which Pb and Cd are among the most toxic. They are very harmful to the human body, even in small quantities. Heavy metals lead to various disorders of a woman's reproductive system such as infertility, delayed puberty, hormonal disorders and gynecologic cancers. Recently, calixarenes and their thiacalixarene derivatives are considered promising agents against heavy metal poisoning and chemosensors of metal cations due to their exceptional ability to form complexes with these ions. It was first determined that the thiacalyx[4] arenes C-800 eliminates the inhibitory effect of Cd, Pb, Zn and Ni cations on the ATPase of myosin subfragment-1. It was suggested the possible mechanisms of elimination of thiacalyx[4]arenes of inhibitory effects of Cd, Pb, and Zn cations on myosin subfragment-1 using the method of computer simulation. The possibility of using thiacalyx[4]arenes as chelating agents in living systems was studied for the first time on the example of uterine myocytes. The ability of thiacalyx[4]arene C-800 to reduce the negative effect of Cd and Pb cations on the respiratory chain of mitochondrial cells of myometrial cells, namely NADH dehydrogenase and mitochondrial succinate dehydrogenase, was also revealed. It was shown the feasibility of using thiacalyx[4]arene C-800 as a sensitive and selective fluorescent probe to Zn cations.