Bulaievska M. Biotechnology of artificial magnetic labeling and natural magnetic labeling of animal cells

Bulaievska М. Biotechnology of artificial magnetic labeling and natural magnetic labeling of animal cells. – Qualifying scientific work on the rights of the manuscript. Thesis for candidate of Doctor of Philosophy the degree in the specialty 162 Biotechnology and Bioengineering. – National Technical University of Ukraine «Igor Sikorsky Kyiv Polytechnic Institute», Ministry of Education and Science of Ukraine, Kyiv, 2021. The work was done at the Department of Bioinformatics Igor Sikorsky Kyiv Polytechnic Institute. Currently, there are detailed studies of the biomineralization of BMNs by microorganisms, but for multicellular organisms, there are only fragmentary studies of the biomineralization of biogenic magnetic nanoparticles. Magnetic nanoparticles are increasingly used in biomedical applications, from contrast agents for magnetic resonance imaging (MRI) to the destruction of cancer cells through treatment with hyperthermia. Most of these promising programs require well-defined and controlled interactions between magnetic nanoparticles and living cells. In this regard, the urgent task is to study the nanostructural localization of BMNs, their morphology and quantity in various organs and tissues of animals, as well as to study the accumulation of artificial magnetic nanoparticles. Using bioinformatics methods, it was shown that among animals, namely non-migratory fishes, the producers of BMNs are common carp and northern pike. Organs and tissues of Atlantic salmon and silver carp were examined for the presence of BMNs. The main regularities of spatial and morphological properties of BMNs in different tissues and organs of animals are determined. The process of artificial magnetic labeling of animal cells was studied. A method for detecting and isolating cells with natural and artificial magnetic properties is proposed, which reduces the time spent on detecting and isolating cells with natural and artificial magnetic properties by using a system of magnets with a slit that has a simpler design in comparable to analogues. The forces of magnetodipole interaction arising between biogenic magnetic nanoparticles of fish organs and exogenous magnetic nanoparticles are calculated. The results of scientific work, which are presented in the thesis, obtained by the author personally or with her scientific advisor participation.