Reheda L. Biological features of Pholiota (Fr.) P. Kumm. species in culture

The dissertation is devoted to studying cultural and morphological, physiological, and biosynthetic characteristics of 18 strains of 8 Pholiota species from the IBK Mushroom Culture Collection, particularly six strains of 3 Pholiota species, isolated by the applicant from carpophores collected in Ukraine. Experimental studies have shown critical incubation temperatures of vegetative mycelia at which strains remain viable. The pH values of the nutrient medium favorable for biomass production for each of the studied species of the Pholiota genus were established. For the first time, information on the accumulation and productivity of endopolysaccharide synthesis in the vegetative mycelia of seven species of this genus, as well as results on the content and productivity of synthesis of triterpene acids of lanostane type in members of the genus. The content and productivity of synthesis of phenolic compounds in biomass and culture fluid of the Pholiota genus were established for the first time. A study of the antimicrobial properties of extracts of biomass, culture fluid, as well as triterpene acids of lanostane type of seven species of this genus. The influence of genus biomass on seed germination, growth of Lepidium sativum and Cucumis sativus seedlings was studied for the first time. The antioxidant activity of biomass extracts and culture fluid of the studied strains of species of the Pholiota genus was determined for the first time. Molecular genetic methods confirmed the species affiliation of 5 strains of 5 species of the Pholiota genus from the IBK Collection and deposited the corresponding molecular sequences in the international database GenBank. The microstructures of vegetative mycelia of eight species of the Pholiota genus were studied, which complement the criteria for assessing the taxonomic affiliation of strains during in vitro storage. Recommendations for the storage of vegetative mycelium of 7 cultures of 7 species of Pholiota in vitro, indicating the conditions of cultivation and biological characteristics of strains.