Meskalo O. Functioning of Ca2+,Mg2+- and Na+,K+-ATРаses in the spermatozoa of men with pathospermia

The dissertation is devoted to actual problems – studying of properties of the basic ion-transporting systems in spermatozoa of men at various forms of pathospermia for the purpose of establishment of functional value and diagnostic value that has essential medico-biological value. A comprehensive study of P-type ATPases (Na+,K+-ATPases, tapsigargin-resistant and tapsigargin-sensitive Ca2 +,Mg2 +-ATPases) in spermatozoa of infertile men with various forms of pathospermia was performed. Inhibition of enzymatic activities of Na+,K+-ATPase, tapsigargin-resistant and tapsigargin-sensitive Ca2 +,Mg2+ -ATPase was found in spermatozoa of men with astheno-, oligoastenozoospermia and leukocytospermia with respect to leukocytospermia. The kinetic properties of Na+,K+-ATPase and tapsigargin-resistant and tapsigargin-sensitive components Ca2+,Mg2+-ATPase of male sperm with pathospermia were studied. It was found that under conditions of H2O2-induced oxidative stress there is a dose-dependent inhibition of the activity of ubain-sensitive Na+,K+ – and Ca2+, Mg2+ -ATPase of sperm. Tapsigargin-resistant component Ca2+, Mg2+ -ATPase of sperm of pathospermian samples is more susceptible to H2O2-induced damage compared to normozoospermic samples. It was found that the calix[4]arenes of C-107, by inhibiting Na+,K+-ATPase, does not change the kinetic parameters of the dependence of the reaction rate (KATP, nH) on the substrate concentration in the sperm of healthy men. However, there is a decrease in the coefficient of cooperation nH inhibition of ATPase reaction of calix[4]arene by C-107 almost twice, in oligo- and asthenozoospermic samples.