Burkot V. Characteristics of the environmental factors influence on gram-negative non-fermenting bacteria′ biologicalactivity

Nowadays, group of gram-negative non-fermenting bacteria includes more than 30 biological species, some of them are dangerous pathogens causing human diseases and their spreading in the etiological structure of opportunistic infections has been steadily increasing in recent years. In this dissertation the biological properties of pseudomonads and acinetobacteria strains isolated from patients with burn and mine wounds investigated. The primary structure of nucleotide sequences of genetic material was determined and antibiotic resistance genes were identified. The conducted multilocus sequence typing allowed to identify among the selected strains of non-fermenting bacteria variants belonging to international clones of high epidemic risk. Thus, 40% of the A. baumannii studied strains belong to the sequence type ST78 according to the Pasteur scheme. Among the P. aeruginosa studied strains were found representatives of two global high-risk clones, namely: ST235 (4 strains) and ST1047 (3 strains). 14 acinetobacteria genetic determinants and 22 pseudomonads ones of the various beta-lactamases production were found in the studied hospital strains. The influence of nutrient media composition, medium pH, cultivation temperature, increased osmotic pressure and reduced atmospheric pressure on the biofilm forming ability of A.baummanii and P.aeruginosa clinical isolates was studied.The results of studies selected strains of P. aeruginosa and A. baumannii showed significant interspecies and interstrainsdifferencesof their ability to film formation.