Korniienko N. Bacterial viruses of the families Enterobacteriaceae and Pseudomonadaceae as potential components of antimicrobial drugs

The dissertation is devoted to the study of lytic properties of specific bacterial viruses (bacteriophages) from families Enterobacteriaceae and Pseudomonadaceae. Selection and characterization of bacteriophages specific to common pathogens of bacteriosis, their biological and genetic properties were studied, the scheme of interaction of phages with bacteria in planta was developed and the influence of phages on the primary link of plant immune response was investigated. The urgency of the topic of qualification work is due to the need to find and study new lytic bacteriophages specific to phytopathogenic bacteria - representatives of the families Enterobacteriaceae and Pseudomonadaceae, which are widespread pathogens of phytobacteriosis in Ukraine. Antimicrobial activity of phages is well studied in vitro, but there is a large gap in in planta studies, both in Ukraine and worldwide. The variety of methods of bacteriophage efficacy research against their hosts in planta is also problematic as the results of experiments cannot be compared. The effect of co-inoculation of bacteriophage and bacteria on the immune response of plants is poorly investigated. The search for bacteriophages specific to bacteriosis pathogens that cause diseases of important plant crops for agriculture is an urgent task for Ukraine, the agricultural sector of which is still important for the economy of both our country and Europe. According to EU standards and requirements for the purity of crop products from chemically and biologically active substances against plant pathogens, antibiotics are not recommended, so the search for alternative approach to fight bacteriosis is an important task for scientists. One of the most effective and promising methods is the use of lytic, pathogen-specific bacteriophages (phages).