Shunxiao L. Molecular mechanism of reproductive isolation of Bemisia tabaci species complex is for plant protection and quarantine services

The study on Bemisia tabaci (Gennadius), an invasive pest that is widespread in tropical, subtropical and other regions of the world, examined its stress resistance, especially in the context of temperature effects. It was noted that high temperature can cause a stress response in B. tabaci, which can affect its development and reproduction. In this study, RACE and insect ecology technologies were used to study the effect of temperature on heat stress protein expression and growth and development characteristics of B. tabaci. It was found that different temperature conditions affect the rate of egg hatching and adult reproduction, while high temperature can lead to a decrease in the reproductive capacity of the pest. The effectiveness of various chemical treatments on B. tabaci, including the use of pesticides, was investigated. It was found that some preparations have a significant control effect on the pest, in particular preparation F and preparation J, which showed high efficiency in a short period of time after treatment. In the genetic aspect, the hsf1 gene and the Hsp60 gene of B. tabaci were studied, and their expression at different temperatures was determined. It has been shown that gene expression correlates with temperature stress, and the Hsp60 gene plays a protective role through the regulatory factors of Hsf1. In conclusion, the study recommends the use of pesticides, such as preparation F and preparation J, for the control of B. tabaci on tomato. The field studies confirm the safety of using these products on vegetable crops. Also, taking into account the genetic aspects, the interaction of hsf1 and Hsp60 genes in the context of the pest's stress response to different temperature conditions was studied. The study indicates the need for further work in the field of B. tabaci control and understanding of its adaptation to temperature changes to improve control strategies for this invasive pest in agriculture.