Scientific novelty of the results obtained. For the first time, it was shown that water loading of negative redox potential with hydrogen saturation compared to induced diuresis of negative redox potential without hydrogen saturation causes recovery of glomerular filtration rate, filtration fraction and proximal reabsorption of sodium ions and reduction of protein losses in urine from in the absence of activation of the mechanism of the basal tone of the vessels of the cortical substance of the kidneys, which is explained by the high permeability of molecular hydrogen and the uniform supply of electrons to all organs and tissues. For the first time, it was established that during the formation of the no-reflow syndrome in rats on a low-sodium diet, 72 hours after the introduction of sublimate, the protective property of molecular hydrogen on the proximal part of the nephron was shown, with an improvement in its functional state, energy supply, an anti-edematous and antioxidant effect was revealed, which promotes the rupture of large and small viciouse circles with an antioxidant solution of molecular hydrogen due to its high permeability and ability to neutralize the hydroxyl radical and peroxynitrite. For the first time, it was shown that in acute hemic hypoxia of moderate severity, the application of a solution of negative redox potential saturated with molecular hydrogen due to its antioxidant, cytoprotective, energetic properties improves the condition of the proximal tubule, reduces the manifestations of tubular proteinuria, and increases the activity of succinate dehydrogenase in this department nephron and reduces the kaliuretic effect of aldosterone by inhibiting the activity of tubulo-glomerular feedback and the intrarenal renin-angiotensin-aldosterone system. It was shown for the first time that the use of a solution of negative redox potential saturated with molecular hydrogen due to its antioxidant, anti-inflammatory, nephroprotective properties has a protective effect on the kidneys and reduces the increased level of tumor necrosis factor-alpha in blood plasma, lysis of azocollagen, azoalbumin, azocasein and products of lipid peroxidation of malonaldehyde and diene conjugates under acute hemic hypoxia of moderate severity. For the first time, it was established that under conditions of kidney hypoxia due to the breakdown of oxidation and phosphorylation processes, the use of a solution of negative redox potential saturated with molecular hydrogen due to its antioxidant, cytoprotective, energetic properties improves the condition of the distal tubule, reduces the manifestations of tubular proteinuria, increases the activity of succinate dehydrogenase in the proximal part of the nephron and reduces the concentration of potassium ions in urine against the background of a decrease in the redox potential of urine to negative values. The first established protective effect of molecular hydrogen for irritable bowel syndrome with constipation and diarrhea leads to the elimination of dysbacteriosis, intoxication syndrome, lipid peroxidation, improves the general condition of patients according to the Likert scale, reduces the degree of proteinuria, and restores the fibrinolytic activity of urine. It was shown for the first time that the protective effect of molecular hydrogen in irritable bowel syndrome with constipation and diarrhea improves the functional state of the proximal tubule of the kidneys due to its high permeability and selective antioxidant activity in neutralizing the hydroxyl radical and peroxynitrite, which contributes to the breaking of large and small vicious circles of the combined pathological process of damage intestines and kidneys.
The practical significance of the results obtained. To improve the functional state, energy potential of the kidneys, provide anti-edematous action, antioxidant effect, rupture of large and small vicious circles during the formation of the no-reflow syndrome in acute kidney damage, it is advisable to use the protective effect of the antioxidant solution of molecular hydrogen on the proximal part of the nephron due to its high permeability and ability to neutralize hydroxyl radical and peroxynitrite. Taking into account the property of the solution of negative redox potential with saturation of molecular hydrogen to improve the functional state of the kidneys under acute hemic hypoxia, it is advisable to use it to improve the functional state of the proximal part of the nephron during this pathological process.
