Liu Z. Construction of multiplex PCR assay based on the citE2 gene to identity Salmonella Pullorum and its effector SteE in pathogenicity and immunity

The thesis is devoted to providing development and scientific justification of the prevention and accurate detection of S. Pullorum, and clarify the mechanism of persistent S. Pullorum infection in chickens. S. Pullorum is a host-specific pathogen, causes severe economic losses to the chicken farms, and also can induce an anti-inflammatory response in chickens. A rapid and accurate method can help us make corresponding prevention and control measures in clinical and food samples. Additionally, S. Pullorum effectors with anti-inflammatory function can be a unique drug candidate for targeting salmonellosis.In order to confirm the pathogenic bacteria species and its drug susceptibility that causes severe diarrhea (pullorum disease) and systemic fatal diseases in chickens. The diseased chickens with suspected S. Pullorum from a large-scale chicken farms were subjected to bacterial culture identification and drug sensitivity test. S. Pullorum were the main pathogenic bacteria of the diseas.