Stroianovska L. Immunobiological reactivity of cows with inflammatory process of the mammary gland under the influence of liposomal preparation based on ethylthiosulfanylate

The dissertation research is aimed at analyzing the spread of mammary gland diseases in cows in the farms of Khmelnitsky region, identifying the microbiota in catarrhal and subclinical mastitis of cows, identifying the sensitivity of the main pathogens to antibacterial drugs, determining the effect of a new complex liposomal preparation based on ethylthiosulfanylate on the activity of antioxidant defense and physiological activity of the cellular and humoral links of natural and adaptive immunity of cows in the clinical and subclinical course of mastitis; the ascertain of employing DNA markers associated with milk SCC as a preventative measure against udder diseases in Ukrainian Black-and-White dairy cows. It has been established that in the clinical course, staphylococci (41.2%) account for the majority of pathogenic microorganisms in associations. In the latent course of the disease, representatives of the genus Streptococcus (26.5%) and Staphylococcus (23.5%) predominate. Treatment of sick cows with a liposomal preparation based on ethylthiosulfanylate (ETS) was accompanied by positive changes in blood morphology, contributed to a decrease in the intensity of oxidation processes and an increase in the activation of protective mechanisms of the antioxidant system, there was a decrease in the number of "zero" rosette-forming T-lymphocytes (TE-ROL), active rosette-forming T-lymphocytes (TA-ROL) and theophylline-resistant lymphocytes (Th-ROL) in both forms of the disease. At the same time, there was a slight increase in the proportion of T-suppressors in the blood of cows: in the catarrhal form of mastitis by 2.2%, in the subclinical form - by 2%. After the use of the therapeutic substance, the proportion of active B-lymphocytes (with low and medium avidity) increased by 2.7% (P < 0.05). An intensive increase in bactericidal activity by 9% (P < 0.01) and lysozyme activity by 6.2% (P < 0.01) on day 7 of treatment in cows with catarrhal mastitis and by 11.4% (P < 0.01) and 11% (P < 0.001) in cows with latent mastitis was found. It was found that the BoLA-DRB3.2*24 allele has an association with a low content of somatic cells in milk, and the BoLA-DRB3.2*22 allele with a high level of CSC.