Berzenina O. Biochemical speciality surface lipids of Coniferae leaves.

Українська версія

Thesis for the degree of Candidate of Sciences (CSc)

State registration number

0400U001892

Applicant for

Specialization

  • 03.00.04 - Біохімія

29-06-2000

Specialized Academic Board

Д 26.001.24

Taras Shevchenko National University of Kyiv

Essay

Тhe disertation is dedicated to study of surface lipids of Coniferous plants leaves in the process of the needle development. The methodical approach for study surface lipids has been worked out. The trends of change of compounds in content of surface lipids were shown for all species of studied needle plants. Meanwhile the generic and specific peculiarities have been observed. The dinamic changes in the carbohydrate fraction concerning the elevation of heterogenity level were found. The increase of the part of highmolecular components were noticed. The fraction of fatty acids contained high level of odd-carbon fatty acids. During the process of the needle development the high increase of behenic acid content was observed, besides the level of even-carbon acids content decreased. These data obtained shown general tend regularity in changes of fatty acid fraction of surface lipids for all studied species. Using the method of differential thermal analysis (DTA) the presence of two phases transitions w ithout mass loss has been shown. There transitions could be classified as surface lipids melting points of amorphous and crystalline phases respectively. The method of purification of considerable amount of fatty acids with long chain from modified extract of deciduous needle on the base of obtained results has been proposed. This method could be recommended for practical use.

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