Fomenko I. Peculiarities of glutamate dehydrogenase and aminotransferases reactions in the intestinal tract mucosa of fetus

The object - fetuses of cows. The aim - to determine the changes in activity of glutamate dehydrogenase, glutamine synthetase and aminotransferases reactions of mucosa in rumen, reticulum, omasum, abomasum, caecum, rectum and liver of the cow fetuses during prenatal period of ontogenesis. The methods - spectrophotometricly, enzymaticly, chromatografy. The results - The preparations of glutamate dehydrogenase from tissue of the fetal rumen mucosa and two forms of glutamate dehydrogenase from rumen mucosa of mature animal, such as tissue form and bacterial form, have been obtained. Glutamate dehydrogenase activity is very low in mitochondria of mucosa in every compartment of the fetal gastrointestinal tract. Activity of aminotransferases in cellular compartments (as cytosol-microsomal and mitochondrial fractions) of mucosa of the whole gastrointestinal tract is on the high level and it becomes higher by age of fetus. The additional load of digestive system by ammonia nitrogen causes increasing of glutami ne synthetase and aminotransferase reactions and increasing of concentration of nitrogen-containing intermediates under decreased content of ATP in rumen mucosa of pregnant cow and fetus. The new introduction - for the first time the use of rape-seed meal for the pregnant cows has been motivated. The degree of introduction - the proposals have been introduced into practice. The sphere of using - biochemistry, veterinary medicine, animal husbandry