Semenov Y. Spectral properties of indo-1 at binding with Ca2+in the cell and protein's solutions

Українська версія

Thesis for the degree of Candidate of Sciences (CSc)

State registration number

0402U001544

Thesis Registration Form

0402U001544.pdf

Applicant for

Specialization

  • 03.00.12 - Фізіологія рослин

14-11-2002

Specialized Academic Board

Д 26.001.38

Taras Shevchenko National University of Kyiv

Essay

The spectral properties of indo-1 in the cell and protein's solutions as well as the influence of the temperature and hyper potassium solution upon rat thymocytes' [Ca2+]i were investigated by the method of spectral analysis. It was determined that Kd og indo-1 decreases more than twice upon the temperature increase from 15 to 37(С. The enthalpy of Ca2+ binding to indo-1 was found and it was shown that (Нdoesn't vary in protein's solutions. Than it was shown that there is electronic excitation energy transfer from proteins to indo-1 in protein's solutions and thymocytes. The effectiveness of electronic excitation energy transfer from proteins to indo-1 was found for the solutions of serum albumines.

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