Kapustian A. Isozyme composition of peroxidase under the low temperature stress

The investigation of transformations of cereal isozyme system showed directional change in activity and isozyme composition of different plants. The increase in intensity of winter-resistant wheat and rye existent peroxidase zones with comparative electrophoretic mobility (Rf 0.14, 0.20) was determined. For the first time the system of differential half-quantitative detection of specific m-RNA for pox3 and pox 4 genes of Triticum aestivum was worked out. This method was recommended for functional detection of plant winter-resistance. The level of peroxidase gene expression was determined by m-RNA level of different winter-resistant winter cereals under the low temperature stress correlated with their gene expression. The methods of prognostication of winter-resistance of cereals by the peroxidase markers was proposed.