Lukashov S. Сyanines with the steric hindrances for fluorescent nucleic acid detection

Cyanines with the steric hindrances, nucleic acids.Development of the approaches to obtaining of new fluorescent dyes by introduction of the substitutents in polymethine chain of trimethinecyanines and study influence of a nature of the substitutents on spectral-luminescent properties of the modified dyes at presence NA. Organic synthesis, spectroscopy, gel-electrophoresis. The C-acylation of active methylene groups of nitrogen heterocycles quaternary salts by carboxylic acids imidazolides is proposed. More than 40 dyes are synthesized and their spectral-luminescent properties in nucleic acids presence are examined. Meso-substitution of trimethinecyanine polymethine chain by primary aliphatic group decrease intrinsic dye fluorescence level, whereas meso-substituted dye fluorescence intensity in nucleic acid complex is in 2-3 times higher than intensity of unsubstituted dye complex luminescence. It was shown that the main way of trimethinecyanine-DNA interaction is intercalation. We propose highly fluorescent trimethinecyanines for use as fluorescent probes for nucleic acids detection. Sphere of use - bioorganic chemistry, molecular biology.