Object of research: Protein metabolism in hens organism of different age by fat feeding. The purpose of work: the research of protein metabolism in different organs and tissues of hens during ontogeny, the role of amino acids, glucose and fat acids in regulation of these processes at different stages of poultry development , the influence of different levels of protein and fat in diet on its growth and on protein metabolism of proteins in organism. The methods of research and the equipment: biochemical and radiological spectrophotometer-16, the automatic amino acids analyzer AAA-Т339, Kieldal apparatus, photoelectrocolometer, pH-meter, densitometer, liquid scintillationary counter LKB. The theoretical and practical results: protein synthesis and degradation intensity and substrate mechanisms of regulation of these processes were investigated. Protein level, their amino acid and fractions composition in liver, skeletal muscles, mucous thin intestine, skin of the hens of egg line at different stages ofgrowth and hatching, influence of fat additions to diet of laying hens on the above-mentioned processes were determined. Use of fats in feeding of laying hens as the components of mixed fodder with the purpose of increase of their body growth and egg productivity was justified. Novelty: intensity of protein synthesis and degradation in different organs and tissues of the hens of egg line at different stages of body growth and hatching, influence of fat additions to diet of the laying hens on the above-mentioned processes in the researched organs and tissues and egg productivity for the first time were investigated. Age dynamics of total level of protein, their amino acids and fractional composition in different organs and tissues of the hens was investigated. The significant increase of protein synthesis intensity in skeletal muscles, skin and mucous thin intestine of the hens in period 30- to 60-days of age and their increase in liver in 30- 180-day's age were determined. Stimulating influence of lysine, palmitic acid and glucose by their addition in incubation medium on protein synthesis in skeletal muscle and skin of hens in 120-day's age was established. The gradual enhancement of acidic proteases activity in and decrease of neutral proteases activity in liver of 30-180-day's age hens, enhancement of both proteases activities in skeletal muscles in 30-60-day's age hens were determined. The decrease of an alanine and aspartataminotransferases activity in skeletal muscles, skin, mucous thin intestine and enhancement of activity aspartataminotransferase in liver of the hens in 60- 120-day's age were determined. It was established, that by feeding of sunflower oil and residual fractions of fish oil as the components to mixed fodder to laying hens protein synthesis intensity increased and neutral proteases activity is decreased, in oviduct - the activity of acidic proteases is decreased. Degree of use: the obtained results will be used at preparation of the recommendation for normalization of hens feeding. The area of use is physiology, biochemistry, poultry-farming, feeding.
