USACH O. Specific features of the effects of tumor-cell based vaccines produced using various techniques on specific and non-specific immune responses (experimental study).

The Thesis deals with studying the effect of anti-tumor vaccines produced on the basis of tumor cells (which were either treated with synthesis products of B. subtilis 7025 or subjected to controlled enzymatic hydrolysis) on the non-specific and adaptive immune responses and provides substantiation of the indicators to be used for purposes of monitoring of the host's response to vaccination. The anti-tumor and anti-metastatic activities of the vaccines studied are based on the induction of cytotoxic lymphocytes, antibody-dependent cell-mediated cytotoxicity, as well as activation of natural killer cells and macrophages. It is proved that B. subtilis 7025 can be cultured using Gause synthetic medium; this helps to standardize the vaccine production technology. Based on the correlation analysis, it has been established that the following immunologic parameters need to be monitored in order to assess the efficacy of the vaccines studied: levels of specific immune responses, functional activity of naturalkiller cells and macrophages, and in vitro IL 2 and TNF production.