Ivanova V. The utilization of the clumping factor of Staphylococcus aureus for diagnostics of the staphylococcal etiology of infections

The serum anti-Clf-antibody levels, their specificity and affinity to Clf in patients with staphylococcal antritis, osteopurulent infection and in healthy individuals, adults and children, were studied by immunoensyme assay (ELISA). Antibody levels against Clf and antibody affinity were shown to be increased during the disease. Diagnostic value of serum anti-Clf-antibodies detection and possibility of the given antigen use for the diagnostics of the staphylococcal infections was demonstrated. Experiments for optimization of the clfA gene expression in E. coli XLI-Blue cells were carried out. The bacterial strain constitutively producing recombinant Clf has been constructed. The ELISA test-system aimed for the detection of Clf-specific antibodies was designed using recombinant Clf. The system was applied at Laboratory of Microbiology and Chemotherapy of Institute of Traumatology and Orthopedics (Academy of Medicine Sciences of Ukraine, Kyiv, Ukraine).