Korotyeyeva G. Viruses and viral diseases of representatives of Orchidaceae Juss. family - biological characteristics and distribution on the territory of Ukraine.

Українська версія

Thesis for the degree of Candidate of Sciences (CSc)

State registration number

0403U003028

Applicant for

Specialization

  • 03.00.06 - Вірусологія

23-09-2003

Specialized Academic Board

Д 26.001.14

Taras Shevchenko National University of Kyiv

Essay

This thesis is dedicated to studying of orchid viruses' characteristics and their distribution in the conditions of glass houses and natural flora of Ukraine. The main viruses of terrestrial and horticulture orchids are determined. The irregular distribution of these viruses has been shown on the territory of Ukraine. Moreover, absence of CymMV and ORSV was determined in the samples of tropical orchids in their natural inhabitances in Vietnam. Tolerant cultivar of Cymbidium hybridum hort. was defined among orchid plants of Ukraine National botanical garden collection. Polyclonal antiserum to the mixture of CymMV and ORSV was obtained to develope diagnosticumbased on indirect ELISA with alkaline phosphatase for detection of these viruses in plant material at different stages of cultivation. Scheme of orchid crop sanitation from viral infection has been modified and technology for propagated terrestrial orchids from seeds in vitro is developed.

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