Teplyuk N. Glutathionetransferase activity and DNA-adducts in human placenta in radiationally and chemically polluted environment

Functional status of detoxifying system and genotoxic damage depending on genotype of detoxifying enzymes were studied in human placenta from radiationally and chemically exposed pregnancies. The negative correlation was revealed between glutathionetransferase activity and the level of DNA-adducts in placenta. The higher glutathionetransferase activity and lipid peroxidation was observed in the carriers of cytochrome P4501A1 (CYP1A1) 462Val isoform comparing 462Ile one. First was determined that benzo(a)pyrene induces expression of both stage detoxication enzymes СYP1A1 and glutathionetransferase Р1 (GSTP1) in human choriocarcinoma cells. Multiple regression analysis was used to evaluate the influence of radiational and chemical exposure from the environment, individual CYP1А1 and GSTP1 genotypes on placental glutathionetransferase activity. It was proposed to use the indices of glutathionetransferase activity and DNA-adduct level in placenta as biomarkers of environmental pollution. Glutathionetransferase activity and genotype of detoxifying enzymes in placenta may be used as a prognostic factors for newborns.