Kyrychenko A. The role of cellular protein-carbohydrate complexs in realization of resistance to tobacco mosaic virus

The dissertation is dedicated to investigation of the role of protein, carbohydrates and their interaction in an initiation and function of resistance mechanism in hypersensitive plants under TMV - infection. It was established that there is no definite correlation between localization of virus infection (LVI) and resistance of leaves to the repeat infection in N. sanderae, D. stramonium and D. metel plants. It was shown that yeast mannans have capability to interfere into realization of virus-induced defense reactions. The antiviral activity of mannans depend on presence and efficiency of infection localization mechanism in different plant species. The date obtained provide evidence for existence of LVI stages in the hypersensitive plants and that these stages may be intensified by exogenous and endogenous glycoconjugates due to protein-carbohydrates interaction. Activity of polysaccharides is blocked by chemically modificated monosaccharides such as -methyl-D-manozide and -methyl-D-glycozide. At the same time native monosaccharides had no effect on them. -Methyl-D-manozides were able to act as supressors blocking LVI mecchanizm and development of induced viral resistance in hypersensitive plants. Change of proteins and carbohydrates contens in dynamic of virus infection for plants with different efficiency of LVI has been studied. It has been established thet TMV-infection produces increase of this components in the appoplast of hypersensitivity plants even in one day after inoculation. The lectin-like proteins were absent in control in hypersensitive plants infected by TMV. The glicoconjugates capable to interact as ligands with mannosa-glucosa containing concanavalin A has been discovered in the intracellular fluid of hypersensitive plants