Saleh J. Androgen function correction in experimеntal animals by transplanting native and cryopreserved testes' organ culture

In dissertation we have performed a comparative analysis of functional characteristics of native and cryopreserved rat testes' organ cultures and ones of new born piglets in vitro and in vivo. Object androgen function of native and cryopreserved testes organ cultures in animals. The work is aimed on comparative analysis of functional characteristics of native and cryopreserved rat testes' organ cultures and the ones of new born piglets in vitro under allo- and xenotransplantation to animals with hypogonadism. Methods and apparatus: organ culture, method of 2 stage freezing, colorimetric and radiometric methods; program freezer "Cryoson", (-counter, needle Dufo. Theoretical and practical results: comparative analysis of the state of native and cryopreserved rat testes' organ cultures and of new born piglets in vitro and in vivo under allo- and xenotransplantation to orchiectomized animals or animals with hypogonadism induced by the administration of cadmium chloride is presented. An absence of influenceof long term preservation of endocrine material on its morphofunctional characteristics permits to conclude about the possibility of creating the endocrine material reserve that allow to introduce wider the transplantation method of treating testes hypofunction into clinical practice. The new: it has been shown that cryopreservation and long term preservation at -196oC provides the preservation of functional characteristics of endocrine material. Cryopreserved material during its transplantation to experimental animals promote the significant increase in testosterone level of blood plasma. The level of approach: the use of cryopreserved organ cultures of new born piglets as transplants is advisable for correction of endocrinopathy. Field of application: endocrinology, andrology. 6156