Kostenko I. Antigenic and genetical differences variants of viruses of a poliomyelitis

In dissertation work introduced outcomes of researches on obtaining and analysis of properties of hybridomas which produce monoclonal antibodies (MAbs), capable to differentiate dissociants (bentonitic variants) vaccine strains of viruses of a poliomyelitis І, ІІ and ІІІ of a type. The panel MAbs is obtained, with the help to which one in reacting virus neutralisation (RVN) is demonstrated, that dissociants, which one differ on a bentonitic token, have miscellaneous a structure of epitops. The sequenation of pieces of genes is conducted, which one encode core proteins VP1, VP4 and virus protease 2A. One replacement of a nucleotide adenine on guanine arranged in a gene is detected which one encodes protein VP1 in a position 2900 pairs of nucleotides. The construction of aminoacidic sequence has shown, that the given replacement results in replacement of an amino acid asparagine on an aspartic acid in a position 142 core proteins VP1. It in turn influences changes of a common charge of protein and its isoelectric point, and also results in local changes of a conformation of protein, and to changes of some biological properties.