Kopko I. Phosphorus compounds in hen's tissues under action of microbial phytase activity and alimentary factors

Українська версія

Thesis for the degree of Candidate of Sciences (CSc)

State registration number

0405U002046

Applicant for

Specialization

  • 03.00.04 - Біохімія

19-04-2005

Specialized Academic Board

Д 35.368.01

Institute of Animal Biology of NAAS

Essay

Research objec: exchange processes phosphorus compounds in hen's organs under the action of microbial phytase activity alimentary factors. Research purpose: the purpose of work was to investigate the influenсe of microbial phytase, lyzine and sodium metasilicate content while their adding to the diet of laying hens, and the influence of the source of phosphorus compounds and protein in their diet for assimilation of phosphorus in intestine as well and the contence of phosphorus metabolism in their tissues. Research method: biochemical, morphometric, chromatographic, statistic. Scientific novelty: on the basis of the investigations it was established pH: optimumfor for microbial and plant phytases by sodium phytate cleavage in vitro, bioavailability of microbial phytase to phytates degradation in different feeds in vitro and in situ in hen's small intestine and microbial phytase influence by its addition to hen's diet on phosphorus release and phosphorus compounds content in blood and liver. It was revealed the increased concentration of neorganic and lipoid phosphorus, RNA and ATP phosphorus in the blood of laying hens by feeding them the diet with high phosphorus and protein level and lysine.It was established stimulating influence of sodium metasilicate addition to laying hens diet on phosphorus release in intestine and use in phosphorus-content compounds synthesis in their organism. Degree of application: the patent for a way of phosphorus assimilation by laying hens in diet of plant origin. Range of application: physiology, biochemistry, poultry, feeding.

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