Lashchevsky V. New approaches to identification of lactic acid bacteria.

The dissertation is devoted to taxonomic researches of lactic acid bacteria (LAB) stored in the Ukrainian collection of microorganisms (UCM). It was shown that heterogeneity of their phenotypical properties creates significant difficulties for their identification. The proper taxonomic position of strains that haven't clear species definitions were defined by molecular methods and using phenotypic data. It was established the atypical fermentation reactions for some Lactobacillus strains. For LAB definitions, it was presented the numeric analysis data. Also, the restriction endonucleases were selected to reveal heterogeneity of a 16S rDNA for some LAB species. The new approach was introduced into identification of LAB at species level. It was based on application of RAPD-primer and the primer-"terminator" and allowed to supply genetic characteristics of strains. The analysis of 16S rDNA sequences of LAB from GENBANK was confirmed data to reference to identification of closely related species using 16SrDNA sequences and shown the limits of 16S-23S spacer region. The PCR express-system was developed for identification of industrially important L. plantarum strains. The special computer program was written by C++ Builder. It was used in computing appurtenant to preparation of solution, PCR and et.al.