Sichkar S. Carbohydrates of surface structures of cells of Mollicutes and phylogenetically related to them genera of Bacillus - Lactobacillus - Streptococcus

Surface polyglicane complexes (glycocalyxes) of some representatives of a Mollicutes class and genera of Bacillus, Lactobacillus and Streptococcus were studied. Glycocalyxes of some representatives of a Mollicutes, Bacillus and Lactobacillus were allocated. Their monosaccharide composition was investigated with electron-microscopic and biochemical methods. And the immunochemical properties of surface cellular antigens of the representatives of these genera were investigated too. Shown the wide spectrum of carbohydrates, which differs depending on the genera and the strains of microorganisms, by method of visualization of extracellular carbohydrate components with the plants' lectins marked with colloid gold. Glycocalyxes of some representatives of Mollicutes and phylogenetically related to them genera of Bacillus, Lactobacillus, Streptococcus were investigated by biochemical methods. Shown the presence of a wide spectrum of carbohydrates (rhamnose, fucose, ribose, arabinose, xylose, mannose, galactose,glucose, galactosamine, glucosamine), in which quantitative structure glucose (10.1-41.3%) and galactose (27.4-49.4%) were dominant. Reserch of immunochemical activity by the methods of Ouchterloni of double immunodiffusion in agar and reverse reaction of radial immunodiffusionmong investigated strains, revealed common antigens with good reaction. The titers of antibodyforming are determinated too. The results of serological methods of investigations can be used as additional attribute at study of phylogenetically related genera of microorganisms.