Rakhimova O. Biological characteristics of natural biocoenosis Myxobacteria from Ukraine south.

The thesis is dedicated to isolation and identification of myxobacteria from various natural habitat of the Ukraine south and studying of their biological characteristics. The data about morphological, physiological, biochemical, molecular-biological characteristics of fresh isolated and museum strains are shown in the thesis; the antibiotic resistance, ability to antagonism, adhesion and heavy metal accumulation are investigated; the rules of long-term conservation of myxobacterium strain are found out. The quantitative and species structure myxobacterium of soil, sea water, and alive plant, soil from zone of splash and seawater from zone of splash was determined. It was shown that myxobacteria are widely spread in soil, seawater, plant sample, and soil from zone of splash and seawater from zone of splash of Ukraine south. It has been carried out for the first time that gliding bacteria which form fruiting bodies were found out in the soil from zone of splash and seawater from zone of splash with 60 %frequency of occurrence. These bacteria have possibility to grow with 15 % NaCl. The diagnostic scheme for Myxococcales member identification on the family level has been carried out for the first time. This diagnostic scheme bases on physiological and biochemical characteristics: Congo red adsorption, oxidase and urease activity, starch hydrolyzes, agar penetration and nitrate reduction. It was shown that the best method for preservation proteolytic and cellulolytic myxobacteria is cryoconservation. The biorhythm in the life cycle myxobacteria was found out for the first time. This fact gave possibility to determine favorable condition for reconservation and investigation of myxobacteria on the basis of discovered rules.