Krysenko A. Peculiarities of catabolic processes in antibiotics resistant Neisseria gonorrhoeae strains

The dissertation is devoted to a research of glucose catabolism pathways, central metabolism and structural and functional peculiarities of membranes in sensitive and resistant to antibiotics Neisseria gonorrhoeae strains. It is shown that multiresistant gonococcus strains are characterized by intensification of reactions of Entner-Doudoroff pathway and glycolysis, with intensity of the oxidative link of pentose phosphate cycle decreasing at the same time. In the strains specified the 2-keto-3-deoxy-6-phosphogluconate aldolase activity is 25,8 - 34,5 % higher, phosphofructokinase activity is 25,3 - 28,9 % higher and fructose 1,6-diphosphate aldolase activity is 26,9 - 34,5 % higher, the 6-phosphogluconate dehydrogenase activity is 19,0 - 27,8 % lower. There has been observed a decrease in citrate synthase activity by 28,9 - 35,0 % and dehydrogenases of isocitrate, 2-ketoglutarate and succinate by 18,8 - 39,3 %, and also a decrease of acetate and some tricarboxylic acid cycle (TCA cycle) substrates oxidation rate in multiresistant strains, on a background of rising activity of fumarase (by 25,7 - 40,6 %), enzymes catalyzing anapleurotic reactions (malic enzyme and PEP-carboxylase by 36,3 - 48,7 %) and enzymes of a constructive metabolism connected with TCA cycle (glutamate dehydrogenase by 70,1 - 87,3 %, aspartate aminotransferase by 32,1 - 46,0 %), which testifies to intensified orientation of TCA cycle to biosynthetic needs. It is shown that breathing rate at the presence of some exogenous substrates was inhibited by KCN (by 72,4±2,1 - 72,9±3,3 % at the maximum), rotenone only inhibited NADH oxidation (by 63,3±2,4 % at the maximum). There have been noticed no differences in sensitivity to the given inhibitors in the strains investigated. The lipid/protein index (0,25±0,01 - 0,39±0,02) of membranous preparations of the given strains has been determined. The majority of antibiotic resistant strains have a 1,16 - 1,56 times higher index compared to the sensitive strain, which testifies to an extra content ofa membrane lipid component.