Leonova N. Physiological activity of Bradyrhizobium japonicum and efficiency of soybean-rhizobia symbiosis in the presence of phytoregulators

The dissertation is devoted to the research of B. japonicum physiological activity and efficiency of their symbiosis with soybean under the phytoregulators activity. The optimal concentrations of phytoregulators for the intensification of the rhizobia growth and the improvement of the quality of the bacterial fertilizer based on B. japonicum 71т were determined. It has been established that after the cultivation of high efficient strain B. japonicum 71т with isoflavonoid daidzein the accumulation of biomass and exopolymers increases and the activity of ammonium assimilation enzymes (glutamine synthetase and glutamate dehydrogenase). At the first time it has been investigated that flavonoids and plants growth regulators don't produce a significantly impact on the components of soybean nodule bacteria exopolymers. The influence of the plants growth regulators on the forming and functioning of soybean-rhizobia symbiosis has been studied. It has been shown that the increasing of crops can be achieved due to the optimal selection of symbiotic partners and plants growth regulators. It is revealed that the application of synthetic regulator Ivin and natural regulator - Emistim C without inoculation and Emistim C and composition preparation - Agrostimulin in conditions of inoculation by high efficient strain B. japonicum 71т indicted the increasing of symbiosis productivity. The suggested methods give the possibility to use the potential of various preparations in order to create high efficient soybean-rhizobia systems.