Artemenko O. Influence of hydrogen peroxide on calcium concentration in rats tymocytes

The dissertation is devoted to research of free calcium concentration in cytosol and mitochondrion and pH cytosol at early stages of action of different hydrogen peroxide concentration in a range from 10 up to 100 mM. It is shown, that at growth of temperature from 12° С up to 32°С the effect of action Н2О2 and speed of accumulation of ions Са2 + in cytosol increases at 12 times. Differences are established in action of small concentration peroxide hydrogen 10 mkM and 100 mkM on change [Ca2 +] in suspension of rats tymocytes. It is established, that Н2О2 causes accumulation of ions Са2 + in mitochondrion tymocytes and increase the concentration of calcium in mitochondrion tymocytes at action of different Н2О2 concentration has doze dependence. It is shown, that endoplasmic reticulum plays an in-significant role in endocellular liberation of Са2+ ions at action of Н2О2. It is shown, that adding to cells digitonin (5 mkM) increasing of the intensity of fluorescence a rhod-2 that testifies fast accumulation of ions Са2 + in mitochondrion. Protonophor the CCCP (2 mkM) leads to fast reduction of concentration of Са2 + ions in mitochondrion.