Object of research: biological macromolecules: DNA, lysozyme, collagen, DOPC, thymine molecule, total lipid fraction isolated from tumour tissues, cell cultures before and after exposure of the nanostructures with different nature and antitumor drugs, tissue sections. Objective: to determine spectroscopic markers and molecular mechanisms of different nanostructures influence on the conformational properties of biological molecules, as well as on the morphological and functional properties of cells and tissues and to identify the relationship between spectral markers and conformational and structural properties of studied objects. Research methods: infrared spectroscopy (FTIR), Raman spectroscopy, coherent anti-Stokes Raman spectroscopy (CARS), confocal microscopy, MTT - colorimetric test, fluorescence spectroscopy, quantum chemical calculations. Practical results: spectroscopic markers of biological macromolecules, cells, and tissues' interactions with antitumor drugs and nanostructures were established, as well as relationships between spectral markers and conformational properties of studied biological objects were established for the purpose of practical application in biophysics, biology, biochemistry, medicine, and diagnostics, and for fundamental research. Scientific novelty: For the first time, the protective impact of 2D-BN nanoparticles in the complex with doxorubicin was demonstrated. It was shown the possibility to use the CARS spectroscopy to visualize biomolecules and carbon nanostructures. It was shown that multilayer graphene can be used as a substrate for infrared spectroscopy with enhancement properties. It was found the gel-phase in the total fraction of phospholipids from a resistant strain of tumour cells at room temperature. It was found a set of spectroscopic markers for conformational analysis of the secondary structure of collagen in different types of tissues. Practical significance: application for studying molecular mechanisms of interaction of biomolecules with different nanostructures and drugs.
