Olishevsky S. Enhancement of the cancer glycopeptide vaccine efficacy using CpG DNA of Bacillus subtilis (experimental studies)

The present work is focused on the development of novel adjuvant based on bacterial DNA. Results of studies directed on investigation of immunobiological effects of novel adjuvant and research of the efficacy of its immunotherapeutic application alone or combined with cancer glycopeptide vaccine are provided and discussed in detail. Bacterial DNA was isolated from Bacillus subtilis culture medium, identified and characterized. The presented results have demonstrated that in vitro and in vivo applications of B. subtilis CpG DNA were associated with positive modulation of functional activity of innate immunity cells, strong induction of interferon ?/? and significant stimulation of cellular and humoral immune responses. Significant anticancer and antimetastatic effects of B. subtilis CpG DNA were obtained. The possibility of application CpG DNA from B. subtilis as an adjuvant for enhancement of cancer glycopeptide vaccine efficacy was proved in experiments.