Investigation object: changes of lipid composition, processes of peroxide oxidation of components cardiomyocytes plasma membranes and blood cells (erythrocytes and thrombocytes) under the experimental hypercholesterolemia and stress. The aim: to reveal state features of cardiomyocytes and blood cells plasma membranes under the effect of experimental hypercholesterolemia and stress, роsibility pharmacologic correction this changes on the early stages of experimental pathology. The methods: biochemical's (the differential ultracentrifugione, spectrophotometric and spectrophluorimetric methods, definition contents of peroxide oxidation products of lipids (POL) and proteins in membrane of sarcolemma, blood cells and in serum, the level of gexoses and sial acids, the density of (-adrenoreceptors, cholesterol and phospholipids contents, Na+,K+- ATPase activity, catalase (CAT) and superoxide dismutase (SOD) activities were investigated), statistics. Results, novelty of investigation: for the first time,impairments were shown on the early stages of experimental hypercholesterolemia. These experimental pathology (hypercholesterolemia and stress) is determined to be accompanied by some stereotypic quantitative and qualitative modifications occurred in the surfase of the cardiomyocytes plasma membranes (decrease of level of gexoses and sial acids) and in the lipid matrix of the membrane structures of cardiomyocytes and blood cells - increase of cholesterol content, decrease of phospholipids. These modifications are also accompanied by the infringement of density of (-adrenoreceptors.There are demonstrated results that the experimental stress has an atherogenic effect on the plasma membranes of cells by inputting the cholesterol into the membrane even in the intact animals with normal lipid metabolism. All these modifications are also accompanied by the activation of free-radical oxidation of lipids and proteins and development the oxidative stress. All these changes are capable to lie in the basis of physicaland chemical properties mechanism modification of membranes: modification of lipid matrix, change of viscosity, ion-transport properties of cardiomyocytes membranes, oppression of Na+,K+- ATPase activity. For the first time, the research marks that diltiasem and captopril has an membranoprotective effects under the experimental hypercholesterolemia.Degree of application: these findings may be used in the complex of measures directed to the prevention and correction changes of physical and chemical properties of cardiomyocytes membranes, modification its lipid matrix, change of viscosity, ion-transport properties on the early stages of atherosclerosis. The results of research diltiasem and captopril influence may be used in the complex therapy in cardiology. Area of application: medical biochemistry, Health Care.
