Chervatyuk N. Functional incompatibility of autonomous genetic elements in Erwinia carotovora.

First, it was described that cells of E. carotovora subsp. carotovora RC5297 and M2-4/50RI produce the unique enzyme complex with the endo- and exonuclease activity. The endonuclease activity of cells lysates becomes apparent at 8 - 14 ?С, and exonuclease activity becomes apparent at 28 - 37 ?С. It was described that exogenous pKM101 plasmid that contains ard gene which overcomes I type restriction E. coli doesn't influence on the ZF40 plating efficient. Independent limitation of ZF40 phage productive growth by the restriction-modification system of exogenous plasmid pECL18 and homing RM-system of strain RC5297 was shown. For research modification restriction Е. carotovora the original system on the basis of virulent polyvalent bacteriophages FE44 and T7, specific rather enterobacteria were offered to updating. It was shown, that exogenous plasmid R68.45 raises lysogeny stability at participation of phage ZF40 and and destabilizes defective lysogeny of E. carotovora. For the first time it was shown, that transformation of E. carotovora cells was influenced with an enzyme complex, which nuclease activity depends on a stage of bacteria growth.