Vasylyshyn Z. Laboratory diagnostics of tick born encephalitis and Lyme disease among the population of endemic territories

A research object is the efficiency of serological method - of the enzyme immunoassay (ELISA) with comparison of different producers' kits for the Tick-borne encephalitis (TBE) diagnostics and verification of the Lyme diseases (LD) diagnosis; aim: to improve the laboratory diagnostics of TBE and LD at pre-hospital and hospital stages of medical-prophylactic service granting to the seasonal unknown fever states patients; research methods: serological - ELISA for the detection of IgM and IgG against TBE viruses and borrelies in blood serums of the fever state patients and healthy persons; molecular-genetic - polymerasechain reaction; descriptive & estimative, analytical, statistic methods; theoretical and practical results - it is proved criteria of syndromal supervision and the indication to diagnosis verification tick-borne diseases - TBE and LD. It is defined criteria and terms of selection of blood sera of seasonal patients with a fever for serological inspections and possibility of use of test systems for ELISA in Ukraine. It is spent an estimation of risks of a lesion of the population by originators TBE and LD and it is developed methodical approaches to their laboratory diagnostics among the population of endemic territories; novelty - the comparative study of different producers of diagnostic kits for the ELISA was firstly conducted for the serological diagnostics of TBE and LD with estimation of their fitness to the using in Ukraine. The laboratory investigation of seasonal fever state patients which had corresponded to the standards of definition of the case of diseases by ELISA allowed to set the TBE and LD prevalence in the structure of the seasonal fever states on territory of Transcarpathian region and define the clinical & epidemiological features of LD and TBE on a modern stage, including the indexes of prevalence, morbidity, features of the territorial distributing and risk groups, that were exposures by agents that were transmitted by ticks. Scientifically substantiated and developed the methodical approaches of laboratory diagnostics of tick-borne infections - TBE and LD on all stages of granting of medical-prophylactic service for the population of Ukraine; introduction degree: results of work are introduced in educational process of medical HIGH SCHOOLS of Ukraine and in practice of sanitary-and-epidemiologic service; branch is a virology.