Bilyavs'ka L. Biosynthesis of antiparasitic and phytostimulating compounds by Streptomyces avermitilis UCM Аc-2179

The thesis is devoted to study of Streptomyces avermitilis UCM Ac-2179 ability to synthe size antiparasitic antibiotic avermectin and phytostimulating compounds, and to research their biological activity. It has been selected highly productive strain S. avermitilis UCM Ac-2179 which ability to synthesize of avermectin tops the level of initial strain 13,3 times as high. Antiparasitic preparation avercom with content 1400-1800 mkg of avermectin per 1 ml is received on its basis. Synthesis of avermectin by S. avermitilis UCM Ac-2179 is increased under carrying pyruvate, treonine and methionine in the cultivation medium in 2,5, 1,6 and 1,3 times accordingly, and decreased in the prechol of valine or isoleicine.It is shown that synthesis of avermectin and lipids by strain-producer are closely connected. It is demonstrated, for the first time, that S. avermitilis UCM Ac-2179 able to produce de novo not only avermectin but in addition the complex of matters with phytostimulationg and phytohormonal action: aminoacids, lipids, including fatty acids, and phytohormonas: auxin, gibberellins and cytokinins. S. avermitilis UCM Ac-2179 reveals antagonisticaetion to some phytopathogenic fungi and stimulats the growth of soil saprophytic bacteria. Among them are B. Megaterium and А. chroococcum which are the basis of phospobacterin and azotobacterin. These bacteria used jointly with avercom favoure to increase the member of pedotrophic, ammonifying, amilolytic and phospatmobilizing bacterian in soil. It has been confirmed thehigh avercom nematocidic efficacy. It is shown, for the first time, that plant growth regulators emictim C and biolan intensify avercom nematocidic action. It is shown that avercom prolons the term of production vegetation of cucumber plant, diminishs the number of increases crop capacity under closed soil.